Improved methods for transducing cells

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/85 (2006.01) A61K 38/19 (2006.01) A61K 48/00 (2006.01) C07K 1/00 (2006.01) C07K 14/47 (2006.01) C07K 14/565 (2006.01) C12N 15/86 (2006.01) C12Q 1/68 (2006.01) C12Q 1/70 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2275438

Disclosed are compositions and methods for inhibiting the expression and/or activity of endogenous .beta.-interferon in cells targeted for transduction with viral vectors, particularly adenoviral vectors. Therefore, also provided are improved methods for treatment of genetically-based diseases by gene therapy. Also disclosed are methods for the treatment of neovascularization- related diseases, for examples, cancer, by the production in vivo of angiostatin, which inhibits the formation of new blood vessels. In particular embodiments, this is accomplished by transduction of macrophages ex vivo with a GM-CSF gene, thereby inducing the secretion of macrophage metalloelastase, which converts plasminogen to angiostatin. The transduced macrophages, when administered, naturally home to tumor sites to effectively localize the therapeutic effect.

Compositions et méthodes pour inhiber l'expression et/ou l'activité de l'interféron-.beta. endogène dans des cellules ciblées pour une transduction avec des vecteurs viraux, en particulier avec des vecteurs d'adénovirus. L'invention concerne par conséquent également des méthodes de thérapie génique améliorées pour des maladies génétiques. L'invention porte également sur des méthodes de traitement des maladies liées à une néovascularisation, par exemple le cancer, par la production in vivo d'angiostatine inhibant la formation nouveaux vaisseaux sanguins. Dans des modes de réalisation particuliers, ce résultat est obtenu par transduction de macrophages ex vivo avec un gène GM-CSF, induisant la sécrétion de métallo-élastase de macrophage, laquelle convertit le plasminogène en angiostatine. Une fois administrés, les macrophages transduits se dirigent naturellement vers les sites tumoraux, réalisant un ciblage efficace de l'effet thérapeutique.

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