G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/68 (2006.01) G01N 33/00 (2006.01) H01J 49/04 (2006.01) H01J 49/26 (2006.01)
Patent
CA 2303758
A method of identifying a protein, polypeptide or peptide by means of mass spectrometry and especially by tandem mass spectrometry is disclosed. The method preferably models the fragmentation of a peptide or protein in a tandem mass spectrometer to facilitate comparison with an experimentally determined spectrum. A fragmentation model is used which takes account of all possible fragmentation pathways which a particular sequence of amino acids may undergo. A peptide or protein may be identified by comparing an experimentally determined mass spectrum with spectra predicted using such a fragmentation model from a library of known peptides or proteins. Alternatively, a de novo method of determining the amino acid sequence of an unknown peptide using such a fragmentation model may be used.
Micromass Limited
Micromass Uk Limited
Ridout & Maybee Llp
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