Improved monocyte activation test better able to detect...

Details Improved monocyte activation test better able to detect... Improved monocyte activation test better able to detect...

G - Physics

01

N

G01N 33/68 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2633523

An improved monocyte activation test is described that is better able to detect non-endotoxin pyrogens in medical products, in which a sample is incubated with a monocyte-containing reagent in an assay system comprising at least one surface comprising polypropylene. The invention also concerns assay systems for use in these tests that include at least one microtiter well having at least one interior surface comprising polypropylene and having a shape such that monocyte-containing reagent is concentrated in the well to provide greater cell to cell contact. The invention also relates to a diagnostic kit that can be used to test for the presence of non-endotoxin pyrogens in a sample.

L'invention concerne un test d'activation de monocytes amélioré, qui permet de mieux détecter des pyrogènes non endotoxiniques dans des produits médicaux, selon lequel un échantillon est incubé avec un réactif contenant des monocytes dans un système de dosage comprenant au moins une surface contenant du polypropylène. L'invention concerne également des systèmes de dosage, destinés à être utilisés dans ces tests, qui comprennent au moins un puits de microtitration ayant au moins une surface intérieure contenant du polypropylène et ayant une forme telle que le réactif contenant des monocytes se concentre dans le puits de façon à assurer un plus grand contact entre les cellules. L'invention concerne en outre un kit de diagnostic qui peut être utilisé pour rechercher la présence de pyrogènes non endotoxiniques dans un échantillon.

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