Improved mutants of (2,5-dkg) reductase

Details Improved mutants of (2,5-dkg) reductase Improved mutants of (2,5-dkg) reductase

C - Chemistry, Metallurgy

12

N

C12N 15/53 (2006.01) C12N 1/21 (2006.01) C12N 9/04 (2006.01)

Patent

CA 2242965

Mutants of 2,5-diketo-D-gluconic acid reductase A and B, enzymes used to produce 2-keto-L-gulonic acid, a precursor of ascorbic acid (vitamin C), are prepared by site-directed mutagenesis. These mutants may exhibit one or more of the following characteristics: improved temperature stability, increased resistance to substrate inhibition, increased turnover of the substrate by the enzyme and increased affinity for the substrate.

La présente invention concerne la préparation, par mutagenèse dirigée sur un site, de mutants de réductase A et B d'acide 2,5-dicéto-D-gluconique, c'est-à-dire des enzymes servant à la production de l'acide 2-céto-L-gulonique qui est un précurseur de l'acide ascorbique (vitamine C). Ces mutants peuvent présenter l'une au moins des caractéristiques suivantes: stabilité thermique améliorée, résistance accrue à l'inhibition du substrat, exploitation plus importante du substrat par l'enzyme et affinité accrue pour le substrat.

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