C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 1/15 (2006.01) C12N 15/00 (2006.01) C12P 7/64 (2006.01) C12N 15/54 (2006.01)
Patent
CA 2761589
Described are engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing an oil comprising greater than 50 weight percent of eicosapentaenoic acid ["EPA"], an .omega.-3 polyunsaturated fatty acid, measured as a weight percent of total fatty acids ["% TFAs"] and having a ratio of at least 3.1 of EPA % TFAs, to linoleic acid, measured as % TFAs. These strains over-express at least one .about.9 elongase/.about.8 desaturase multizyme, in addition to other heterologous .about.9 elongases, .about.8 desaturases, .about.5 desaturases, .about.17 desaturases, .about.12 desaturases, C16/18 elongases, and optionally over-express diacylglycerol cholinephosphotransferases, malonyl CoA synthetases and/or acyl-CoA lysophospholipid acyltransferases. The expression of at least one peroxisome biogenesis factor protein is down-regulated. Methods for producing EPA within said host cells, oils obtained from the cells, and products therefrom are claimed.
L'invention porte sur des souches modifiées par génie génétique de la levure oléagineuse Yarrowia lipolytica capable de produire une huile comprenant plus de 50 pour cent en poids d'acide éicosapentaénoïque [« EPA »], un acide gras ?-3 polyinsaturé, mesuré en pourcentage en poids des acides gras totaux [« % de TFA »] et ayant un rapport d'au moins 3.1 de EPA % TFA, sur l'acide linoléique, mesuré en tant que % de TFA. Ces souches surexpriment au moins une multizyme ?9 élongase/?8 désaturase, en plus d'autres ?9 élongases, ?8 désaturases, ?5 désaturases, ?17 désaturases, ?12 désaturases, C16/18 élongases hétérologues et facultativement surexpriment des diacylglycérol cholinephosphotransférases, des malonyl CoA synthétases et/ou des acyl-CoA lysophospholipide acyltransférases. L'expression d'au moins une protéine de facteur de biogenèse de peroxisome est régulée de manière négative. L'invention porte sur des procédés pour produire de l'EPA dans lesdites cellules hôtes, sur des huiles obtenues à partir des cellules et sur les produits de celles-ci.
Hong Seung-Pyo
Sharpe Pamela L.
Xue Zhixiong
Yadav Narendra S.
Zhu Qun
E. I. Du Pont de Nemours And Company
Torys Llp
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