Improved peptide identification and quantitation by merging...

Details Improved peptide identification and quantitation by merging... Improved peptide identification and quantitation by merging...

G - Physics

01

N

G01N 33/68 (2006.01)

Patent

CA 2713446

The present invention is directed to methods of merging spectral data resulting from collision fragmentation pro-cesses, such as, for example, Pulsed Q Dissociation (PQD), high-energy collision-induced dissociation (HCD), electron transfer disassociation (ETD), collision-induced dissociation (CID), and photo- dissociation processes, such as, but not limited to, infrared multi-photon photo-dissociation (IRMPD), to provide the desired qualitative and quantitative information on a single peptide. By merging such ETD, CID, or IRMPD scans with corresponding HCD scans that are obtained on the same precursor, the quality of the resulting spectrum is increased so as to provide more confident identification of peptides and correspondingly the quantifica-tion is enhanced because the HCD method of the MS/ MS spectrum produces higher abundances of detectable reporter ions. Such methods, as disclosed herein, are especially applicable for peptides which experience predominant neutral loss in the ion trap, e.g., phosphorylated.

La présente invention concerne des procédés de fusion de données spectrales résultant de processus de fragmentation par collision tels que, par exemple, des processus de dissociation pulsée Q (PQD), de dissociation induite par collision de haute énergie (HCD), de dissociation par transfert délectrons (ETD), de dissociation induite par collision (CID) et de photo-dissociation telle que, sans sy limiter, la photo-dissociation multiphotonique infrarouge (IRMPD), pour fournir les informations qualitatives et quantitatives souhaitées concernant un peptide unique. Par fusion de tels scans ETD, CID ou IRMPD avec des scans HCD correspondants qui sont obtenus sur le même précurseur, la qualité du spectre obtenu est augmentée de façon à assurer une identification plus fiable de peptides et par conséquent, la quantification est amplifiée, car le procédé HCD du spectre SM/SM produit de plus hautes abondances dions rapporteurs détectables. De tels procédés, tels que réalisés ici, sappliquent spécialement à des peptides qui font lobjet dune perte neutre prédominante dans le piège à ions, par exemple qui sont phosphorylés.

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