Improved phytases

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 9/16 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2356642

This invention relates to improved phytases, preferably phytases of an increased thermostability, and a process of producing them. In particular, stabilizing amino acid mutations are introduced into a homologous protein, or the active site of a phytase is replaced in part or in total. The corresponding DNA sequences and methods of preparing them are also disclosed, as are methods of producing the improved phytases, and the use thereof. Specific variants of Aspergillus fumigatus phytase and of consensus phytases are disclosed.

Cette invention se rapporte à des phytases améliorées, telles que de préférence des phytases ayant une thermostabilité accrue, ainsi qu'un procédé de production correspondant. Des mutations d'acides aminés stabilisantes sont plus particulièrement introduites dans une protéine homologue, où le site actif d'une phytase est remplacé partiellement ou totalement. Les séquences d'ADN correspondantes et les procédés de préparation de ces séquences sont également présentées, ainsi que des procédés de production de ces phytases améliorées et leur utilisation. Des variants spécifiques de phytase d'Aspergillus fumigatus et de phytases de consensus sont présentés.

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