Improved primer extension reactions

Details Improved primer extension reactions Improved primer extension reactions

C - Chemistry, Metallurgy

12

Q

C12Q 1/00 (2006.01) C12N 9/16 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2050276

A kit or solution for use in extension of an oligonucleotide primer having a first.single-stranded region on a template mole- cule having a second single-stranded region homologous to the first single- stranded region; comprising a first agent able to cause extension of the first single-stranded region of the primer on the second single-stranded region of the template in a reaction mix- ture, and a second agent able to reduce the amount of pyrophosphate in the reaction mixture below the amount produced during the extension in the absence of the second agent.

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