Improved process for the culturing of cells

Details Improved process for the culturing of cells Improved process for the culturing of cells

C - Chemistry, Metallurgy

12

M

C12M 1/12 (2006.01) C12M 3/06 (2006.01) C12N 5/10 (2006.01) C12N 15/00 (2006.01) C12P 1/00 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2657040

The invention relates to a process for the culturing of cells, preferably E1-immortalized HER cells, more preferably PER.C6 cells in a reactor in suspension in a cell culture medium, wherein the cells produce a biological substance, preferably an antibody, wherein at least one cell culture medium component is fed to the cell culture and wherein the cell culture comprising the cells, the biological substance and cell culture medium is circulated over a separation system and wherein the separation system separates the biological substance from substances having a lower molecular weight than the biological substance and wherein the biological substance is retained in or fed back into the reactor. Preferably part of the substances of lower molecular weight is continuously removed from the cell culture.

L'invention concerne un processus de culture de cellules, de préférence des cellules HER E1 immortalisées, et de préférence encore, des cellules PER.C6, dans un réacteur en suspension dans un milieu de culture cellulaire. Les cellules produisent une substance biologique, de préférence un anticorps. Au moins un composant du milieu de culture cellulaire alimente la culture cellulaire, ladite culture cellulaire renfermant les cellules, la substance biologique et on fait passer le milieu de culture cellulaire par un système de séparation, lequel système de séparation sépare la substance biologique des substances ayant un poids moléculaire inférieur à celui de la substance biologique, ladite substance biologique étant retenue ou réintroduite dans le réacteur. De préférence, une partie des substances de poids moléculaire inférieur est continuellement prélevée de la culture cellulaire.

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