Improved process for the production of l-aspartic acid

C - Chemistry – Metallurgy – 12 – P

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C12P 13/20 (2006.01)

Patent

CA 2269770

A process for production of L-aspartic acid comprising (1) contacting (A) an enzyme-containing material having maleate isomerase activity and aspartase activity, or (B) an enzyme-containing material having maleate isomerase activity and an enzyme-containing material having aspartase activity, with an aqueous substrate solution containing maleic acid and ammonia, and/or mono or di-ammonium maleate, to form L-ammonium aspartate, in a reaction solution, (2) adding the reaction solution to an aqueous solution of maleic acid or maleic anhydride at a controlled rate to crystallize L-aspartic acid in a mother liquor, and (3) recovering the L-aspartic acid from the mother liquor, and (4) optionally recycling said mother liquor as a substrate solution for further reaction with said enzyme containing material of Step 1 and (5) optionally adding alkali such as ammonia to said mother liquor before, during, or after recycle.

La présente invention concerne un procédé de production d'acide L-aspartique consistant (1) à mettre en contact (A) une matière contenant une enzyme à activité maléate isomérase et à activité aspartase ou (B) une matière contenant une enzyme à activité maléate isomérase et à activité aspartase avec une solution substrat aqueuse contenant de l'acide maléique et de l'ammoniac et/ou du maléate de mono-ammonium ou de di-ammionium de façon à former du L-aspartate d'ammonium dans une solution réactive, (2) à ajouter la solution réactive à une solution acqueuse d'acide maléique ou d'anhydre maléique, à une vitesse régulée de façon à cristalliser l'acide L-aspartique dans une liqueur mère, (3) à récupérer l'acide L-aspartique de la liqueur mère, (4) à recycler éventuellement ladite liqueur mère en solution substrat pour une réaction ultérieure avec la matière contenant ladite enzyme de l'étape (1) et (5) à ajouter éventuellement un alcali tel que de l'ammoniac à la liqueur mère avant, pendant et après le recyclage.

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