Improved promoters and utilization thereof

Details Improved promoters and utilization thereof Improved promoters and utilization thereof

C - Chemistry, Metallurgy

12

N

C12N 15/11 (2006.01) A01H 5/00 (2006.01) C12N 5/10 (2006.01) C12N 15/67 (2006.01) C12N 15/82 (2006.01) C12N 15/83 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2401554

Promoters having been improved so as not to undergo methylation in the course of constructing transformants. The above promoters are DNAs as defined by the following (1) or (2): (1) DNAs containing the base sequences represented by SEQ ID NOS:1 to 4; and (2) DNAs containing base sequences derived from the base sequences represented by SEQ ID NOS:1 to 4 by deletion, addition or insertion of one to several bases, being free from the consecutive sequences represented by CG, CAG, CTG, CCG and CGG in the deleted, added or inserted base sequence, and having a promoter activity. By using these promoters, the expression efficiency of a structural gene of a plant showing weak expression even by using cauliflower mosaic virus 35S promoter, which has been considered as a high-expression promoter for plants, (for example, chrysanthemum) can be elevated.

L'invention concerne des promoteurs améliorés de façon à ne pas se méthyler pendant la construction de transformants. Ces promoteurs sont des ADN définis comme suit : 1) ADN contenant les séquences de base représentées par SEQ ID NOS : 1 à 4 ; et 2) ADN contenant les séquences de base obtenues à partir des séquences de base représentées par SEQ ID NOS : 1 à 4 par délétion, addition ou insertion d'une ou de plusieurs bases, exempt des séquences consécutives représentées par CG, CAG, CTG, CCG et CGG dans la séquence de base comportant délétion, addition ou insertion, et possédant une activité de promoteur. A l'aide de ces promoteurs, on peut accroître l'efficacité d'expression d'un gène de structure d'une plante présentant une expression faible, même en présence du promoteur de virus de la mosaïque du chou-fleur 35S, considéré comme promoteur de forte expression chez les plantes (p. ex. chrysanthème).

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