Improved strain for butanol production with increased...

Details Improved strain for butanol production with increased... Improved strain for butanol production with increased...

C - Chemistry, Metallurgy

12

N

C12N 1/21 (2006.01) C12N 1/16 (2006.01) C12N 1/32 (2006.01)

Patent

CA 2745394

Bacteria that are not natural butanol producers were found to have increased tolerance to butanol when the membrane content of unsaturated trans fatty acids was increased. Feeding cells with unsaturated trans fatty acids increased their concentration in the membrane, which may also be accomplished by expressing a fatty acid cistrans isomerase.

L'invention porte sur des bactéries qui ne sont pas des producteurs naturels de butanol dont on a trouvé qu'elles présentaient une tolérance accrue au butanol lorsque la teneur en acides gras insaturés trans dans la membrane était accrue. Le fait de nourrir des cellules avec des acides gras insaturés trans augmenta leur concentration dans la membrane, ce qui peut également être réalisé par l'expression d'une cis-trans isomérase d'acides gras.

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