Improved strains of e. coli for plasmid dna production

Details Improved strains of e. coli for plasmid dna production Improved strains of e. coli for plasmid dna production

C - Chemistry, Metallurgy

12

P

C12P 19/34 (2006.01)

Patent

CA 2577419

A general method and strains of bacteria are described, by means of which it is possible to dramatically purify plasmid DNA with respect to genomic DNA, and RNA. In one preferred embodiment, lysis and nuclease removal of host nucleic acids is an integral component of the fermentation/harvest process, and as such, achieves increased yield and purity with simplified downstream purification and reduced waste streams, thus reducing production costs.

La présente invention concerne un procédé général et des souches de bactéries, au moyen desquels il est possible de purifier de façon très importante l'ADN plasmidique par rapport à l'ADN génomique et l'ARN. Dans un mode de réalisation préféré, la lyse et la suppression des nucléases des acides nucléiques hôtes sont totalement intégrés au processus de fermentation/récolte, ce qui fait que ce procédé donne de meilleurs résultats de rendement et de pureté, tout en simplifiant la purification en aval, et en réduisant les courants d'effluents, et par conséquent les coûts de production.

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