C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01)
Patent
CA 2525482
The invention is directed to a system for performing multi-color real time PCT, comprising a flexible real time PCT instrument and a specific composition or reaction mixture for performing multiplex PCR: In particular, the present invention is directed to a composition or reaction mixtuer which comprises at least 3, preferably 4-5 and most preferably exactly 4 pairs of FRET hybridization probes. Each pair of said hybridization probes consists of a FRET donor probe carrying a FRET donor moiety and a FRET acceptor probe carrying a FRET acceptor moiety having an emission maximum between 550 and 710 nm.
L'invention concerne un système de mise en oeuvre de PCR en temps réel multicolore. Ledit système comprend un instrument de PCR en temps réel flexible et une composition ou un mélange réactionnel spécifiques pour la mise en oeuvre d'une PCR multiplex. La présente invention concerne notamment une composition ou un mélange réactionnel qui comprend au moins 3, de préférence entre 4 et 5 et idéalement exactement 4 paires de sondes d'hybridation FRET. Chaque paire desdites sondes d'hybridation est constituée d'une sonde donneur FRET portant un fragment donneur FRET et d'une sonde accepteur FRET portant un fragment accepteur FRET présentant une émission maximum située entre 550 et 710 nm.
Bechler Ingrid
Bolte Jochen
Gutekunst Martin
Heindl Dieter
Josel Hans-Peter
F. Hoffmann-La Roche Ag
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
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