Improved targeted dna insertion in plants

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/82 (2006.01) C12N 9/22 (2006.01)

Patent

CA 2545564

Methods and means are provided to improve targeted DNA insertion in plants using rare-cleaving "double stranded break" inducing enzymes. Also provided are improved I-SceI encoding nucleotide sequences.

L'invention concerne des méthodes et des moyens destinés à améliorer l'insertion d'ADN ciblée dans des plantes au moyen d'enzymes à sites de clivage rares induisant la "cassure bicaténaire". L'invention concerne également des séquences nucléotidiques codant pour I-SceI.

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