C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/87 (2006.01) A61K 48/00 (2006.01) C12N 5/10 (2006.01)
Patent
CA 2451389
The present invention provides an improved method for gene delivery in eukaryotic cells by electroporation, preferably in human hematopoietic cells, particular dendritic cells. The method of the invention is superior to lipofection and passive pulsing of mRNA and to electroporation of plasmid cDNA for gene delivery, including tumor antigen loading of dendritic cells.
La présente invention concerne une technique améliorée d'apport de gène dans des cellules eucaryotes par électroporation, de préférence dans des cellules hématopoïétiques humaines, en particulier des cellules dendritiques. La technique de cette invention est supérieure à la lipofection et à l'impulsion passive d'ARNm et à l'électroporation d'ADNc de plasmide destiné à l'apport de gène, le chargement d'antigène tumoral de cellules dendritiques y compris.
Berneman Zwi N.
Ponsaerts Peter
Schuler Gerold
Strobel Isolde
Van Tendeloo Viggo F.i.
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
N.v. Antwerpes Innovatiecentrum
Schuler Gerold
University Of Antwerp
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