Improved transformant and process for producing polyester...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/52 (2006.01) C12N 1/19 (2006.01) C12P 7/62 (2006.01)

Patent

CA 2523984

A gene expression cassette comprising an Aeromonas caviae-origin polyhydroxyalkanoic acid (PHA) synthase gene having a mutation introduced thereinto and a promoter and a terminator acting in a yeast; a transformant constructed by transferring the above gene expression cassette into a yeast; and a process for producing a polyester using the above transformant. Thus, it becomes possible to efficiently produce a copolymerized polyester having a high biodegradability and excellent properties by copolymerizing 3- hydroxyaclanoic acids in a yeast.

La présente invention concerne une cassette d'expression génique qui comprend un gène synthase d'acide polyhydroxyalcanoïque provenant de Aeromonas caviae dans lequel une mutation a été introduite, ainsi qu'un promoteur et un terminateur à l'oeuvre dans une levure; un transformant construit par transfert de ladite cassette d'expression génique dans une levure; et un procédé de production d'un polyester au moyen dudit transformant. On peut ainsi produire efficacement un polyester copolymérisé présentant une forte biodégradabilité et d'excellentes propriétés par la copolymérisation d'acides 3-hydroxyalcanoïques dans une levure.

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