Improved transformation and regeneration of wheat using...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/82 (2006.01) A01H 4/00 (2006.01) A01H 5/00 (2006.01) B01F 17/00 (2006.01) C07F 1/08 (2006.01) C12N 5/14 (2006.01) C12N 15/63 (2006.01)

Patent

CA 2447796

A regeneration medium for use in wheat transformation which utilises copper at a concentration in a range from greater than 50 mM to less than or equal to 300 mM and a growth hormone at a concentration in a range from 0.05 to 10 mg l- 1 are disclosed. Growth hormone is added to the regeneration medium if wheat tissue or cell has not been treated with a growth hormone after callus induction and prior to using the wheat regeneration medium. A method for producing a transformed wheat plant using this regeneration medium is also disclosed.

L'invention concerne un milieu de régénération destiné à être utilisé dans la transformation du blé, lequel met en oeuvre du cuivre dans une concentration supérieure à 50 µM et égale ou inférieure à 300 µM ainsi qu'une hormone de croissance dans une concentration comprise entre 0,05 et 10 mg l-1. L'hormone de croissance est ajoutée au milieu de régénération si le tissu ou les cellules de blé n'ont pas été traités avec une hormone de croissance après l'induction de cals avant d'utiliser ce milieu de régénération du blé. L'invention concerne également un procédé de production d'une plante de blé transformée à l'aide de ce milieu de régénération.

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