Improved transformation efficiency in phage display through...

Details Improved transformation efficiency in phage display through... Improved transformation efficiency in phage display through...

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C07K 14/01 (2006.01) C12N 1/00 (2006.01) C12N 15/62 (2006.01) C12N 15/86 (2006.01)

Patent

CA 2334170

The transformation yield of electroporation is increased by using higher DNA concentrations and DNA affinity purification. Fusion proteins of a viral coat protein variant and a heterologous polypeptide are useful in phage display systems.

On peut améliorer l'efficacité de transformation d'une électroporation en utilisant des concentrations d'ADN plus importantes et une purification par affinité de l'ADN. Les protéines de fusion d'un variant d'une protéine de coque virale et d'un polypeptide hétérologue sont utiles dans des systèmes d'expression à la surface des phages.

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