C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/58 (2006.01) C12N 15/81 (2006.01)
Patent
CA 2756752
The present invention provides an improved type protease which comprises an amino acid sequence that is at least 75% identical to SEQ ID NO:3, said improved type protease has at least one mutation selected from the group consisting of. (A) replacement of glutamine corresponding to glutamine at position 265 in SEQ ID NO: 3 with an acidic amino acid; and (B) replacement of glutamine at position 266 in SEQ ID NO: 3 with an acidic amino acid, and wherein said improved type protease has milk-clotting activity.
La présente invention propose une protéase de type amélioré, qui comprend une séquence d'acides aminés, qui est identique à au moins 75 % à SEQ ID N°3. Ladite protéase de type amélioré présente au moins une mutation, choisie parmi le groupe consistant en : (A) le remplacement de la glutamine correspondant à la glutamine en position 265 de SEQ ID N°3, par un acide aminé acide ; et (B) le remplacement de la glutamine en position 266 de SEQ ID N°3, par un acide aminé acide, ladite protéase de type amélioré ayant une activité de coagulation du lait.
Harada Kazunori
Kato Shigeaki
Kobayashi Hiroyuki
Suga Taro
Tsunoda Akira
Meito Sangyo Co. Ltd.
Smart & Biggar
LandOfFree
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