Improved variants of the bacillus licheniformis alpha-amylase

Details Improved variants of the bacillus licheniformis alpha-amylase Improved variants of the bacillus licheniformis alpha-amylase

C - Chemistry, Metallurgy

12

N

C12N 9/28 (2006.01) C12N 9/42 (2006.01)

Patent

CA 2689629

Variants of B. licheniformis alpha-amylase advantageously exhibit improved enzymatic performance. Suitable variants include those with an altered charge distribution on the surface of the enzyme or with altered active site residues. Structural modeling can inform the choice of amino acid modifications so that modified amino acids correspond to residues found in more active alpha amylases, for example. Compositions comprising the variants are useful in methods of cleaning surfaces, laundering textiles, desizing, treating starch, e.g., liquefaction and saccharification, and hydrolyzing biofilms off various substrates.

L'invention concerne de variatns de l'alpha-amylase B. licheniformis qui présetnent de manièr avantageuse un meilleur rendement énergétique. des variants appropriés comprennent ceux ayant une répartition de charge modifiée sur la surface de l'enzyme ou des résidus de sites actifs modifiés. Une modélisaiton strucutrale peut donner le chois des modificaitons d'0acides aminés de sorte que les acides aminés modifiés correspondent aux résidus trouvés dans plusieurs alpha amylases actives, par exemple, des compositions compernant els variants sont utiles dans des procédés de nettoyage de surface, de blabhicssge de textiles, de désencollage, de taitemetn de l'amidon, par exemple, la liquéfaction et la saccharification, et l'hydrolyse de biofilms en dehors de divers substrats.

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