Improved vectors and yeast strains for protein production:...

C - Chemistry – Metallurgy – 12 – P

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C12P 21/00 (2006.01) C12N 5/10 (2006.01) C12N 15/81 (2006.01)

Patent

CA 2733567

Lower eukaryote host cells in which an endogenous or heterologous Ca2+ ATPase is overexpressed are described. Also described are lower eukaryote host cells in which a calreticulin and/or ERp57 protein are overexpressed. These host cells are useful for pro-ducing recombinant glycoproteins that have reduced O-glycosylation.

L'invention porte sur des cellules hôtes eucaryotes inférieures dans lesquelles une Ca2+ ATPase endogène ou hétérologue est surexprimée. L'invention porte également sur des cellules hôtes eucaryotes inférieures dans lesquelles une calréticuline et/ou une protéine ERp57 sont surexprimées. Ces cellules hôtes sont utiles pour produire des glucoprotéines recombinantes qui ont une O-glycosylation réduite.

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