C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/85 (2006.01) A01K 67/027 (2006.01)
Patent
CA 2200465
The invention relates to a method of enhancing the transcription of a gene in a DNA construct incorporated into the genome of a eucaryotic host cell, which DNA construct comprises a structural gene for a desired protein or polypeptide and a gene promoter upstream of the structural gene. The invention resides in providing at least one enhancer element comprising the nucleotide sequence TTC TGA GAA upstream of said promoter, and exposing the DNA construct to lactogenic stimuli. The invention also relates to an expression vector, and a host cell and a transgenic mammal containing this vector.
L'invention se rapporte à un procédé d'amélioration de la transcription d'un gène dans un ADN de recombinaison incorporé dans le génome d'une cellule eucaryote hôte, cet ADN de recombinaison comprenant un gène de structure pour un polypeptide ou une protéine requise et un promoteur de gène en amont du gène de structure. Le procédé selon l'invention consiste à utiliser au moins un élément de renforcement comprenant la séquence nucléotidique TTC TGA GAA en amont dudit promoteur, et à exposer l'ADN de recombinaison à des stimuli lactogènes. L'invention se rapporte en outre à un vecteur d'expression, et à une cellule hôte et un mammifère transgénique contenant ce vecteur.
Enberg Bertil
Haldosen Lars-Arne
Lobie Peter
Norstedt Gunnar
Sliva Daniel
Biovitrum Ab
Fetherstonhaugh & Co.
Pharmacia & Upjohn Ab
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