Improvements for combinatorial oligonucleotide pcr

Details Improvements for combinatorial oligonucleotide pcr Improvements for combinatorial oligonucleotide pcr

C - Chemistry, Metallurgy

12

P

C12P 19/34 (2006.01) C07H 21/02 (2006.01) C12N 9/00 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2488330

The present invention is directed to a method of detecting gene expression and analysis of both known and unknown genes. In the method utilizing combinatorial oligonucleotide PCRTM, no single molecular species of DNA gives rise to more than one fragment in a collection of products which are subsequently amplified and representative of each expressed gene. More specifically, the present invention improves known methods by eliminating the recovery of restriction fragments with two identical ends and furthermore regards amplification and isolation of the restriction fragment utilizing a labeled primer.

La présente invention concerne une méthode permettant de détecter l'expression de gènes et d'analyser des gènes connus et inconnus. Cette méthode fait intervenir une PCR oligonucléotidique combinatoire (combinatorial oligonuclotide PCR.TM.), dans laquelle aucune espèce moléculaire d'ADN ne fournit plus d'un fragment dans un ensemble de produits par la suite amplifiés et représentatifs de chaque gène exprimé. La présente invention concerne plus précisément, d'une part, des améliorations portant sur des méthodes connues, consistant à éliminer la récupération de fragments de restriction comprenant deux extrémités identiques, et, d'autre part, l'amplification et l'isolement du fragment de restriction à l'aide d'une amorce marquée.

Affiliated with

Also associated with

Rating

Improvements for combinatorial oligonucleotide pcr does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Improvements for combinatorial oligonucleotide pcr, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Improvements for combinatorial oligonucleotide pcr will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1958908