C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C07K 14/35 (2006.01) C12N 15/11 (2006.01) C12N 15/31 (2006.01)
Patent
CA 2480351
The present invention relates to the discovery of a DNA sequence in sheep types of M. paratuberculosis that differs from the homologous sequence in cattle types of M. paratuberculosis. The invention also provides a nucleic acid amplification technique based on these differences that can be used to distinguish strains of the cattle type from strains of both the sheep types of M. paratuberculosis. The invention also relates to use of these sequences in a nucleic acid amplification technique to distinguish all strains of M. paratuberculosis from other strains of the MAI complex and from strains of the M. tuberculosis complex.
L'invention concerne la découverte d'une séquence d'ADN dans des types mouton de M. paratuberculosis différant de la séquence homologue dans des types bétail de M. paratuberculosis. L'invention concerne également une technique d'amplification d'acides nucléiques fondée sur ces différences et pouvant être utilisée pour distinguer des souches du type bétail des souches des deux types mouton de M. paratuberculosis. L'invention concerne enfin l'utilisation de ces séquences dans une technique d'amplification d'acides nucléiques permettant de distinguer toutes les souches de M. paratuberculosis d'autres souches du complexe MAI et des souches du complexe M. tuberculosis.
Agresearch Limited
Marks & Clerk
LandOfFree
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