Improvements in and relating to scanning confocal microscopy

G - Physics – 02 – B

Patent

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G02B 21/00 (2006.01) G02B 26/08 (2006.01)

Patent

CA 2663597

An assembly (100) and a method are provided for inputting a light beam into a light path extending from the confocal scanning head (102) to the microscope (104) of a scanning confocal microscope system to illuminate a selected region of a sample mounted in the microscope. The assembly comprises a light input for receiving a light beam from a light source; beam directing means (8, 12) for controlling the path of the light beam with reference to the shape of the selected region of the sample; and a beam coupler (16) for selective coupling the light beam into the light path from the confocal scanning head (102) to the microscope (104), with the beam direction being controlled by the beam directing means so as to illuminate the selected region. A scanning confocal microscope system including such an assembly is also described, together with a method of calibrating the system. In addition, an optical switch for selectively switching a light beam between two paths is disclosed.

Ensemble (100) et procédé permettant d'introduire un faisceau lumineux dans un trajet lumineux allant d'une tête de balayage confocale (102) au microscope (104) d'un système de microscopie confocale à balayage afin d'illuminer une région sélectionnée d'un échantillon monté dans le microscope. L'ensemble comprend une entrée de lumière conçue pour recevoir un faisceau lumineux émanant d'une source lumineuse; des moyens (8, 12) d'orientation de faisceau conçus pour réguler le trajet du faisceau lumineux en fonction de la forme de la région sélectionnée de l'échantillon; et un coupleur (16) de faisceau conçu pour coupler sélectivement le faisceau lumineux dans le trajet lumineux allant de la tête de balayage confocale (102) au microscope (104), la direction du faisceau étant régulée par les moyens d'orientation de faisceau de manière à illuminer la région sélectionnée. L'invention concerne également un système de microscopie confocale à balayage comprenant cet ensemble, ainsi qu'un procédé d'étalonnage du système. L'invention concerne par ailleurs un commutateur optique conçu pour commuter sélectivement un faisceau lumineux entre deux trajets.

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