Improvements in or relating to binding assays

Details Improvements in or relating to binding assays Improvements in or relating to binding assays

G - Physics

01

N

G01N 33/554 (2006.01) C12Q 1/68 (2006.01) C12Q 1/70 (2006.01) G01N 33/50 (2006.01) G01N 33/566 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2225706

Disclosed is a method of testing a substance for the ability to affect the formation or oligomerisation of a complex comprising members of a specific binding pair, a first member of the binding pair being present on the surface of a lipid enveloped particle comprising a transferrable label, said first member of the binding pair being capable of binding to a second member of the specific binding pair present on the surface of a cell, the lipid envelope of the particle being capable of fusing with the membrane of the cell so as to transfer the label to the cell, said transfer being inhibited by formation or oligomerisation of a complex between the first and second members of the binding pair, wherein the method comprises reacting the particle and the cell, in the presence of the substance under test, (under conditions which would allow for binding of the first and second members of the specific binding pair in the absence of the substance under test), and detecting the label transferred, if any; together with a composition for use in the assay method of the invention.

L'invention a trait à un procédé qui permet de tester les capacités d'une substance à influer sur la formation d'un complexe, ou sur son oligomérisation, comportant des éléments d'une paire spécifique de liaison, un premier membre de celle-ci se trouvant à la surface d'une particule à enveloppe lipidique comportant une marque susceptible de transfert, ledit premier élément étant capable de se lier à un second élément de la paire spécifique de liaison se trouvant à la surface d'une cellule, l'enveloppe lipidique de la particule étant capable de fusionner avec la membrane de la cellule de façon à transférer la marque à celle-ci, ledit transfert étant entravé par la formation d'un complexe, ou par son oligomérisation, entre le premier et le second élément de la paire de liaison. Ce procédé consiste, d'une part à faire réagir la particule avec la cellule en présence de la substance testée, (dans des conditions qui devraient autoriser une liaison des deux éléments de la paire de liaison spécifique en l'absence de la substance testée), et, d'autre part à détecter, le cas échéant, la marque transférée. Il est également fait état d'une composition à utiliser dans le cadre du procédé d'épreuve selon l'invention.

Affiliated with

Also associated with

Rating

Improvements in or relating to binding assays does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Improvements in or relating to binding assays, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Improvements in or relating to binding assays will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1643506