Improvements in or relating to contrast agents

A - Human Necessities – 61 – K

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A61K 49/00 (2006.01) A61K 49/18 (2006.01) A61K 49/22 (2006.01)

Patent

CA 2144749

Proteinaceous gas- or gas precursor-containing microbubble contrast agents, e.g. for use in ultrasound and/or MR imag- ing, in which the protein matrix is crosslinked by reaction with a bifunctional aldehyde (e.g. a dialdehyde such as glutaraldehyde or an .alpha.,.beta.-unsaturated aldehyde such as acrolein) in an aqueous medium at substantially neutral pH exhibit improved in vivo and storage stabilities, particularly if the matrix is also reacted with a Schiff's base reducing agent such as a borohydride. Modifica- tion of the size distribution of such crosslinked proteinaceous gas-containing contrast agents, e.g. to reduce the mean size of the microbubbles, further enhances their stability and permits preparation of novel contrast agents having a particularly narrow mic- robubble size distribution.

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