Improvements in or relating to displacement assays

G - Physics – 01 – N

Patent

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Details

G01N 33/543 (2006.01) G01N 33/553 (2006.01)

Patent

CA 2309599

Disclosed is a method of detecting the presence of an analyte of interest in the sample, the method comprising the steps of: reversibly immobilising on a first surface a displaceable moiety; exposing the first surface to a sample comprising the analyte of interest, the analyte of interest specifically displacing the displaceable moiety from the first surface; causing the displaceable moiety displaced from the first surface to contact a second surface bearing a capture moiety which specifically binds to the displaceable moiety, so as to capture the displaceable moiety on the second surface, said capture generating a detectable signal; and detecting the signal.

La présente invention concerne un procédé permettant de détecter la présence d'un analyte d'intérêt dans un prélèvement, lequel procédé consiste à immobiliser de façon réversible sur une première surface un fragment ionisable; à exposer ladite première surface à un prélèvement comprenant l'analyte d'intérêt, ledit analyte d'intérêt déplaçant spécifiquement le fragment ionisable de ladite première surface; à mettre en contact le fragment ionisable déplacé de la première surface avec une deuxième surface supportant un fragment de capture qui se lie spécifiquement au fragment ionisable de façon qu'il capture le fragment ionisable sur la deuxième surface, cette capture générant un signal décelable; et à détecter ledit signal.

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