Improvements in or relating to methods of screening substances

Details Improvements in or relating to methods of screening substances Improvements in or relating to methods of screening substances

G - Physics

01

N

G01N 33/554 (2006.01) C12Q 1/68 (2006.01) C12Q 1/70 (2006.01) G01N 33/50 (2006.01) G01N 33/566 (2006.01)

Patent

CA 2225680

Disclosed is a method of screening substances for reduced ability to bind to a specified target molecule. The method involves the display of a first member of a specific binding pair ("sbp") on the surface of a viral display package, and contacting the viral display package with a target cell expressing a receptor molecule and the second member of the sbp in such a way that viral display packages displaying first members of the sbp which have been altered so as to inhibit binding to the second member of the sbp tend to infect target cells more successfully than those display packages displaying first members of the sbp which have unaltered binding affinity for the second member of the sbp. Also disclosed is a kit for use in performing the method of the invention.

Cette invention concerne un procédé permettant la sélection de substances à une faible capacité de liaison à une molécule cible donnée. Ce procédé consiste à exposer un premier élément d'une paire de liaison spécifique ("sbp" specific binding pair) à la surface d'une capside d'exposition virale, et à mettre en contact ladite capside d'exposition virale avec une cellule cible exprimant une molécule réceptrice et le second élément de la paire "sbp" de telle manière que les capsides d'exposition virale exposant les premiers éléments de la paire "sbp", qui ont été modifiés pour empêcher la liaison au second élément de la paire "sbp", tendent à infecter les cellules cibles avec plus de succès que les capsides d'exposition virale exposant les premiers éléments de la paire "sbp" qui possèdent une affinité de liaison non modifiée pour le second élément de la paire "sbp". Cette invention concerne également un kit permettant de mettre en oeuvre le procédé de l'invention.

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