Improvements in or relating to mutagenesis of nucleic acids

Details Improvements in or relating to mutagenesis of nucleic acids Improvements in or relating to mutagenesis of nucleic acids

C - Chemistry, Metallurgy

07

H

C07H 19/23 (2006.01) C07H 19/10 (2006.01) C07H 19/24 (2006.01) C12N 15/10 (2006.01) C12P 19/34 (2006.01) C07H 19/20 (2006.01)

Patent

CA 2232506

The invention concerns novel compounds having defined structural formulae and methods of mutating a nucleic acid sequence, the method comprising replicating a template sequence in the presence of a nucleoside triphosphate analogue in accordance with the invention, so as to form non-identical copies of the template sequence comprising one or more nucleoside triphosphate analogue residues, and a kit for use in performing the method of the invention.

Cette invention se rapporte à de nouveaux composés ayant des formules structurales définies ainsi qu'à des procédés de mutation d'une séquence d'acide nucléique. Un tel procédé consiste à reproduire une séquence matrice en présence d'un analogue de triphosphate nucléosidique conformément à la présente invention, de manière à former des copies non identiques de la séquence matrice comportant au moins un reste d'analogue de triphosphate nucléosidique. L'invention concerne également un matériel servant à mettre en oeuvre ce procédé.

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