Improvements in or relating to separation processes

Details Improvements in or relating to separation processes Improvements in or relating to separation processes

C - Chemistry, Metallurgy

12

N

C12N 5/00 (2006.01) A61K 49/22 (2006.01) B01F 17/00 (2006.01) B03D 1/00 (2006.01) B03D 1/016 (2006.01) B03D 1/02 (2006.01) B03D 1/14 (2006.01) C07K 16/12 (2006.01) C07K 16/28 (2006.01) C12N 5/06 (2006.01) G01N 33/48 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2326386

Separation of target material from a liquid sample is achieved by coupling the target to targetable encapsulated gas microbubbles, allowing the microbubbles and coupled target to float to the surface of the sample to form a floating microbubble/target layer, and separating this layer from the sample. In a positive separation process the microbubbles are then removed from the target, e.g. by bursting. In a negative separation process target-free sample material is recovered following separation of the floating layer. The method may also be used diagnostically to detect the presence of a disease marker in a sample. Novel separation apparatus is also described.

La séparation d'un matériau ciblé et d'un spécimen de liquide consiste à coupler la cible à des bulles microscopiques gazeuses encapsulées et pouvant être ciblées, à laisser les bulles microscopiques et la cible couplée flotter à la surface du spécimen afin de constituer une couche flottante de bulles microscopiques et de matériau ciblé, et à séparer cette couche du spécimen. Dans un procédé de séparation positive, on supprime les bulles microscopiques de la cible, par exemple, par rupture brusque. Dans un procédé de séparation négative, on récupère le matériau du spécimen exempt de cible après la séparation de la couche flottante. On peut également mettre ce procédé en application dans un but diagnostic afin de détecter la présence d'un marqueur de maladie dans un spécimen. L'invention concerne également un nouvel appareil de séparation.

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