Improvements in or relating to specific binding assays

Details Improvements in or relating to specific binding assays Improvements in or relating to specific binding assays

G - Physics

01

N

G01N 33/74 (2006.01) G01N 33/543 (2006.01) G01N 21/75 (2006.01)

Patent

CA 2254108

Disclosed is a method of detecting the presence of an analyte of interest in a sample, the method comprising the steps of: contacting the sample with a solid support having reversibly immobilised thereon either a binding partner having specific binding affinity for the analyte, or an analogue of the analyte; and detecting a reduction in the mass of material immobilised on the solid support, said binding partner or analogue being specifically displaced from the solid support in the presence of the analyte of interest, so as to cause a detectable change in the mass of material immobilised thereon. Also disclosed is a device for performing the method.

L'invention concerne un procédé permettant de détecter la présence d'un analyte à étudier dans un échantillon, qui comprend les étapes suivantes: on met en contact l'échantillon avec un support solide qui porte, immobilisé de manière réversible, soit un partenaire de liaison présentant une spécificité de liaison spécifique pour ledit analyte, soit un analogue de l'analyte; puis on détecte une réduction de la masse du matériau immobilisé sur le support solide. Le partenaire de liaison ou l'analogue est spécifiquement déplacé du support solide en présence de l'analyte à étudier, ce qui provoque une modification détectable de la masse du matériau immobilisé. L'invention concerne également un dispositif permettant de mettre en oeuvre ledit procédé.

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