Improvements in thermal cycler for pcr

B - Operations – Transporting – 01 – L

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B01L 7/00 (2006.01) C12Q 1/68 (2006.01) F25B 21/02 (2006.01) G01R 27/20 (2006.01) G05D 23/19 (2006.01) H01L 35/28 (2006.01) H01L 35/30 (2006.01)

Patent

CA 2285377

An instrument for performing highly accurate PCR employing an assembly, a heated cover and an internal computer. The assembly is made up of a sample block (36), a number of Peltier thermal electric devices (39) and heat sink (34), clamped together. The sample block (36) temperature is changed exclusively by the thermoelectric devices (39) controlled by the computer. The sample block (36) is of low thermal mass and is constructed of silver. The Peltier devices are designed to provide fast temperature excursions over a wide range. The heat sink (34) has a perimeter trench (44) to minimize edge losses and is adjacent to a continuously variable fan. A perimeter heater is used to improve the thermal uniformity across the sample block (36) to approximately +/- 0.2 °C.

L'invention a trait à un appareillage permettant d'effectuer une PCR des plus précise et comportant un ensemble, un couvercle chauffé et un ordinateur interne. L'ensemble est constitué d'un bloc à échantillons (36), d'un certain nombre de dispositifs thermoélectriques à effet Peltier (39) et d'un dissipateur thermique assujetti aux dispositifs. La température du bloc à échantillons (36) est modifiée exclusivement par les dispositifs thermoélectriques (39) commandés par l'ordinateur. La masse thermique du bloc à échantillons (36) fait d'argent est faible. Les dispositifs à effet Peltier sont conçus pour assurer de rapides excursions thermiques sur une gamme étendue. Le dissipateur thermique (34), qui est pourvu d'une tranchée périphérique (44) afin de minimiser les pertes par les parois latérales, est contigu d'un ventilateur variable en continu. On utilise un système de chauffage périphérique pour renforcer l'homogénéité thermique du bloc à échantillons (36) à environ +/- 0.2 DEG C.

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