Improvements relating to encapsulation of bioactive agents

A - Human Necessities – 61 – K

Patent

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Details

A61K 9/16 (2006.01) A61K 9/50 (2006.01) A61K 39/00 (2006.01) A61K 47/34 (2006.01) A61K 48/00 (2006.01) C12N 15/87 (2006.01) A61K 9/00 (2006.01) A61K 9/113 (2006.01) C12N 15/10 (2006.01)

Patent

CA 2331465

Bioactive agent is encapsulated in a polymer microparticle in a (water-in-oil)- in-water emulsion-based method, and using a solvent that comprises ethyl acetate. Also described are microparticles comprising low inherent viscosity (i.v.) PLG, some with i.v. less than 0.5 dl/g, and methods for their preparation. DNA release is modified through use of low i.v. PLG. A particle production method for scale-up uses a blender that avoids excessive shear damage to DNA being encapsulated.

Un agent bioactif est encapsulé dans une microparticule de polymère selon un procédé basé sur l'émulsion (eau dans l'huile) dans l'eau, et en utilisant comme solvant de l'acétate d'éthyle. L'invention concerne également des microparticules comprenant du PLG à faible viscosité inhérente (i.v.), certaines ayant une i.v. inférieure à 0,5 dl/gm, ainsi que des procédés pour leur préparation. La libération d'ADN est modifiée par emploi de PLG à faible i.v. Un procédé de production de particules à grande échelle utilise un mélangeur qui évite d'endommager par cisaillement excessif l'ADN encapsulé.

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