C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/29 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) C07K 14/43 (2006.01) C12N 1/19 (2006.01) C12N 15/62 (2006.01) C12N 15/81 (2006.01) C12N 15/82 (2006.01)
Patent
CA 2307539
The expression of proteins is improved by post-translational transport to a cellular membrane, especially transport to the extracellular space, by the use of membrane-targeting sequences. One specific use of this method is in the expression of the DNA coding sequence for the sweet-protein brazzein: transport to the extracellular space greatly increases the expression levels.
Selon l'invention, l'expression de protéines est améliorée par transport post-traduction vers une membrane cellulaire, en particulier transport vers l'espace extracellulaire, au moyen de séquences de ciblage de membrane. Une utilisation spécifique de ce procédé consiste à réaliser l'expression d'une séquence de codage d'ADN pour la brazzéine, qui est une protéine sucrée, le transport vers l'espace extracellulaire élevant grandement les niveaux d'expression.
Drake Caroline Rachel
Evans Ian Jeffrey
Osborn Rupert William
Ray John Anthony
Na
Syngenta Limited
Zeneca Limited
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