Improvements to methods for preparing equine embryos for...

A - Human Necessities – 01 – N

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A01N 1/02 (2006.01)

Patent

CA 2293620

The invention concerns a method for preparing an equine embryo consisting in, previously to subjecting said embryo to the action of cryoprotectant(s), in subjecting it to a suitable treatment to eliminate the embryonary capsule or to increase said capsule permeability, in order to reinforce the subsequent action of said cryoprotectant(s). Preferably, the embryonary capsule is destroyed or simply made more permeable by a treatment based on collagenase or trypsin.

Le procédé de préparation d'embryon d'équidé en vue de sa cryoconservation consiste: avant de soumettre ledit embryon à l'action d'agent(s) cryoprotecteur(s), à lui faire subir un traitement approprié pour supprimer la capsule embryonnaire ou pour augmenter la perméabilité de cette capsule, en vue de renforcer l'action ultérieure dudit ou desdits agents cryoprotecteurs. De préférence, la capsule embryonnaire est détruite ou simplement rendue plus perméable par un traitement enzymatique à base de collagénase ou de tryspine.

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