Improvements to the fluorescent polymer-qtl approach to...

Details Improvements to the fluorescent polymer-qtl approach to... Improvements to the fluorescent polymer-qtl approach to...

G - Physics

01

N

G01N 33/533 (2006.01) B01J 13/00 (2006.01) B05D 1/18 (2006.01) C12Q 1/68 (2006.01) C12Q 1/70 (2006.01) G01N 21/64 (2006.01) G01N 33/53 (2006.01) G01N 33/531 (2006.01) G01N 33/542 (2006.01) G01N 33/543 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2409512

A chemical composition including a moiety comprising a quencher (Q), a tethering element (T), and a ligand (L) that associates with and quenches a fluorescent polymer is disclosed. When an analyte sample is introduced, the ligand (L) binds to a target biological agent if it is present, thereby causing the QTL molecule to separate from the fluorescent polymer resulting in an increase in detected fluorescence. The same chemistry is advantageously employed in a competitive assay. An electric field can also be applied to separate the QTL molecule from the fluorescent polymer. A method for detecting targets for these methods are also disclosed.

L'invention concerne une composition chimique comprenant un groupe caractéristique composé d'un quencher (Q), d'un élément de fixation (T) et d'un ligand (L), qui s'associe avec un polymère fluorescent et le trempe. Lorsqu'on introduit un échantillon d'analyte, le ligand (L) se lie à un agent biologique cible, s'il est présent, et provoque la séparation de la molécule QTL du polymère fluorescent, ce qui donne lieu à un accroissement de la fluorescence détectée. On utilise le même procédé chimique de façon avantageuse en dosage compétitif. On peut également appliquer un champ électrique pour séparer la molécule QTL du polymère fluorescent. L'invention concerne enfin un technique de détection de cibles pour ces procédés.

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