Improving polynucleotide ligation reactions

Details Improving polynucleotide ligation reactions Improving polynucleotide ligation reactions

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2552858

The method of the invention is useful in quantifying the absolute or relative number of unique molecules present in a sample after carrying out an analysis procedure on the sample, and comprises the steps of: (i) attaching a unique molecular tag to substantially all of the molecules in the sample; (ii) carrying out the analysis procedure using the molecules of the sample; and (iii) on the basis of the molecular tags determining the absolute or relative number of unique molecules present in the original sample which underwent the analysis procedure.

Le procédé de l'invention est destiné à la quantification du nombre relatif ou absolu de molécules uniques présentes dans un échantillon après une procédure d'analyse de l'échantillon. En l'occurrence, on procède en plusieurs étapes. On commence (I) par attacher un marqueur moléculaire unique à sensiblement la totalité des molécules de l'échantillon. On exécute ensuite (ii) la procédure d'analyse en utilisant les molécules de l'échantillon. Enfin, (iii) sur la base des marqueurs moléculaires, on détermine le nombre absolu ou relatif des molécules uniques présentes dans l'échantillon d'origine ayant subi la procédure d'analyse.

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