In planta method for the production of transgenic plants

Details In planta method for the production of transgenic plants In planta method for the production of transgenic plants

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) C12N 5/10 (2006.01)

Patent

CA 2308702

The present invention relates to a method for producing a transgenic plant in which a transforming agent such as a recombinant Agrobacterium or an isolated DNA molecule capable of conferring a desired phenotypic trait is injected into plant tissues using aneedleless injection device. A preferred embodiment of the method provides the precise delivery of the transforming agent to floral tissues of a plant, allowing the direct insertion of the DNA of the transforming agent into germline cells of the plant that develop into seeds. This embodiment provides a more efficient method of transformation and subsequent regeneration of a transgenic plant.

La présente invention concerne un procédé de production d'une plante transgénique dans lequel un agent transformant, tel qu'un Agrobacterium recombinant ou une molécule d'ADN isolée capable de conférer un caractère phénotypique désiré, est injecté dans des tissus végétaux au moyen d'un dispositif à injecter sans aiguilles. Un mode de réalisation préféré permet de placer avec précision l'agent transformant dans des tissus floraux d'une plante, ce qui permet d'insérer directement l'ADN de l'agent transformant dans des cellules germinatives de la plante qui se développent en graines. Ce mode de réalisation fournit un procédé plus efficace de transformation et de régénération subséquente d'une plante transgénique.

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