In planta transformation by embryo imbibition of agrobacterium

Details In planta transformation by embryo imbibition of agrobacterium In planta transformation by embryo imbibition of agrobacterium

A - Human Necessities

01

H

A01H 1/00 (2006.01) A01H 5/00 (2006.01) A01H 7/00 (2006.01) C12N 15/82 (2006.01) C12N 15/87 (2006.01)

Patent

CA 2457479

The present invention provides a method for the preparation of a plant embryo for Agrobacterium-mediated transformation. The method of preparation uses a novel technique including dehydration of the plant embryo. The invention further contemplates the transformation of the prepared plant embryo. The invention further encompasses the regeneration of a plant or plant cell from the transformed plant embryo.

L'invention concerne un procédé permettant de préparer un embryon de plante destiné à une transformation dont médiateur est une agrobactérie. Ce procédé de préparation fait appel à une nouvelle technique faisant intervenir une déshydratation de l'embryon de plante. L'invention concerne en outre la transformation de l'embryon de plante ainsi préparé, ainsi que la régénération d'une plante ou d'une cellule de plante à partir de cet embryon de plante transformé

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