In situ detection of human t-cell leukemia virus type iii

Details In situ detection of human t-cell leukemia virus type iii In situ detection of human t-cell leukemia virus type iii

C - Chemistry, Metallurgy

12

Q

150/9

C12Q 1/70 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 1254118

ABSTRACT In situ hybridization method for the detection of viral RNA or DNA, involving the hybridization of a tissue sample with an 35S-labeled HTLV-III RNA probe. The probe detects HTLV-III in primary, uncultured tissue samples, is highly sensitive, and is relatively fast acting. The RNA probe may be synthesized from cloned HTLV-III DNA inserted into a suitable transcription vector.

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