In situ hybridization method

Details In situ hybridization method In situ hybridization method

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 2116214

A method of identifying the presence of a known target sequence in nucleic acid contained in a fixed cellular or subcellular biological structure. By adding a stable, reporter-labeled RecA/single-stranded probe complex to the cellular or subcellular structure, the target sequence can be effectively labeled by in situ hybridization, allowing the target sequence to be visualized histologically and microscopically or detected by in situ cytometry or cell sorting flow techniques.

Divulgation d'une méthode pour détecter la présence d'une séquence cible connue contenue dans de l'acide nucléique d'une structure biologique cellulaire ou subcellulaire fixée. En ajoutant à cette structure un complexe stable composé de protéine RecA liée à une sonde à simple brin marquée par un reporteur, il est possible de marquer efficacement la séquence cible par hybridation in situ, ce qui permet de voir la séquence cible à l'examen histologique et microscopique ou de la détecter par des techniques de cytométrie en flux ou de tri des cellules in situ.

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