In situ hybridization solution and process

Details In situ hybridization solution and process In situ hybridization solution and process

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) G02B 21/34 (2006.01)

Patent

CA 2217509

A fluorescence in situ hybridization (FISH) procedure and solutions. The entire FISH procedure is fast (15 minutes or less), with the hybridization step occurring in 5 minutes or less. The entire FISH procedure with digoxygenin- or biotin-labeled probes takes approximately 30 minutes. A formamide-free solution (dextran sulfate and glycerol) is provided. An additional solution (10 % dextran sulfate and 20 % formamide) is also provided. Additional solutions are provided for nRNA in situ hybridization whereby the process takes less than 24 hours.

Procédure d'hybridation in situ par fluorescence et solutions adaptées à cette procédure. L'ensemble de cette procédure est rapide (15 minutes ou moins), l'étape d'hybridation s'effectuant en 5 minutes ou moins. L'ensemble de la procédure d'hybridation in situ par fluorescence, avec des sondes marquées par digoxygénine ou par biotine, nécessite environ 30 minutes. Une solution exempte d'acide formique (sulfate de dextrane et de glycérol) est proposée. Une autre solution (10 % de sulfate de dextrane et 20 % d'acide formique) est également proposée. D'autres solutions sont proposées pour une hybridation in situ d'ARNn selon un proccédé nécessitant moins de 24 heures.

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