In vitro assay based on camp levels modulated by gi-coupled...

Details In vitro assay based on camp levels modulated by gi-coupled... In vitro assay based on camp levels modulated by gi-coupled...

C - Chemistry, Metallurgy

12

N

C12N 5/10 (2006.01) C12Q 1/02 (2006.01) C12Q 1/527 (2006.01) G01N 33/50 (2006.01) C12N 9/88 (2006.01)

Patent

CA 2654517

The present invention relates to a reliable and effective in vitro assay based on cAMP levels modulated by Gi-coupled receptors expressed in a selected cell for screening and identifying pharmaceutically effective compounds that specifically interact with and modulate the activity of a cellular receptor.

La présente invention concerne un dosage in vitro fiable et efficace, basé sur les niveaux d'AMPc que modulent des récepteurs couplés à Gi exprimés dans une cellule sélectionnée, pour le criblage et l'identification de composés pharmaceutiquement efficaces interagissant spécifiquement avec un récepteur cellulaire dont ils modulent l'activité.

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