In vitro assay for detecting cell-mediated immune responses

G - Physics – 01 – N

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G01N 33/569 (2006.01) G01N 33/50 (2006.01) G01N 33/53 (2006.01) G01N 33/68 (2006.01)

Patent

CA 1299099

ABSTRACT OF THE DISCLOSURE An in vitro method of detecting a cell-mediated immune response to a specific antigen in a human or animal, comprises the steps of: (i) incubating a whole blood sample from the human or animal with the specific antigen; and (ii) detecting the presence of gamma interferon (.gamma.IFN) released by sensitized lymphocytes in the whole blood sample to indicate a cell-mediated immune response to the specific antigen. A diagnostic kit is also disclosed.

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