In vitro assay for identification of allergenic proteins

Details In vitro assay for identification of allergenic proteins In vitro assay for identification of allergenic proteins

G - Physics

01

N

G01N 33/68 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2621966

The present invention relates to a process for in vitro evaluation, of a potentially allergenic or tissue irritating substance whereby test cells are cultivated in the presence of the substance, and the presence of up regulated genes chosen from G1P2, OASL, IFITl, TRIM22, IFI44L, MXI, RSAD2, IFIT3, IFITMl, IFIT2, SPR, GNB2, XK, IFITM3, C 33.28 HERV-H protein mRNA, IFITM3, XK, GPR15, MTlG, MT1B;MT1A, ADFP, JL8, MTlE, MTlF, MTlH, SLC30A1, SERPINB2, CD83, TncRNA or expression products from them are measured. The invention also regards use of the expression products from one or more of the genes for in vitro analysis of allergy or tissue irritation. It also relates to a probe comprising at least three nucleic acids, preferably 3 - 40, especially 5 - 15 chosen from RNA complementary to the RNA corresponding to any of the genes and the use threof for in vitro analysis of allergy or tissue irritation. Further it regards a reagent kit comprising one or more probes that recognize products produced during the expression of any the genes.

L'invention concerne un procédé d'évaluation in vitro d'une substance potentiellement allergène ou pouvant irriter les tissus. Les cellules d'essai sont cultivées en présence de la substance, et des gènes à régulation positive choisis parmi G1P2, OASL, IFITl, TRIM22, IFI44L, MXI, RSAD2, IFIT3, IFITMl, IFIT2, SPR, GNB2, XK, IFITM3, mARN de la protéine C 33.28 HERV-H, IFITM3, XK, GPR15, MTlG, MT1B; MT1A, ADFP, JL8, MTlE, MTlF, MTlH, SLC30A1, SERPINB2, CD83, TncARN ou des produits d'expression de ceux-ci sont mesurés. L'invention concerne également l'utilisation des produits d'expression d'un ou de plusieurs de ces gènes pour l'analyse in vitro de l'allergie ou de l'irritation de tissus. L'invention concerne en outre une sonde comprenant au moins trois acides nucléiques, de préférence 3 40, plus spécialement 5 10 choisis dans l'ARN complmentaire à l'ARN correspondant à n'importe lequel des gènes et leur utilisation dans l'analyse in vitro de l'allergie ou de l'irritation de tissus. Elle concerne enfin une trousse de réactifs comprenant au moins une sonde qui reconnaît des produits obtenus lors de l'expression de n'importe lequel des gènes.

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