In-vitro bioassay of erythropoietin

Details In-vitro bioassay of erythropoietin In-vitro bioassay of erythropoietin

G - Physics

01

N

150/13

G01N 33/74 (2006.01)

Patent

CA 1263834

ABSTRACT An in vitro bioassay for erythropoietin comprising the steps of preparing a liquid assay mixture from a Manor Cell Preparation incubating the assay mixture in the presence of labeled thymidine to produce a liquid incubation mixture. At the end of an incubation period the cell material is separated from the liquid incubation mixture and the separated cell material is subjected to a quantitative determination of the incorporated labeled thymidine which is proportional to the erythropoietin content of the sample. The Manor Cell Preparation is prepared by inducing anemia in a rabbit, administering actinomycin D to the anemic rabbit and thereafter collecting bone marrow from the rabbit.

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