In vitro cell culture assembly

C - Chemistry – Metallurgy – 12 – M

Patent

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Details

C12M 3/00 (2006.01) C12M 3/06 (2006.01)

Patent

CA 2186209

An assembly for in vitro culture of cells of the present invention includes a culture vessel with at least one well. The well has an open top, a bottom and a wall with an inside surface. The assembly also includes at least one insert sized for placement within the well. The insert has an outside surface, an open end and a closed end. There is a microporous membrane at the closed end of the insert that closes the end and thereby forms a receptacle within the insert. The assembly further includes provisions for adjustably positioning the insert within the well between a first position where the closed end of the insert is a first distance from said bottom of the well and a second position where the closed end of the insert is a second distance from the well bottom.

L'ensemble pour la culture cellulaire in vitro de la présente invention comprend un récipient de culture comportant au moins un puits. Le puits ouvert comporte un fond et une paroi cylindrique ayant une surface intérieure. L'ensemble comprend en outre au moins une pièce amovible qui s'insère dans le puits. Cette pièce amovible a une surface extérieure, une extrémité ouverte et une extrémité fermée. Une membrane microporeuse ferme l'extrémité fermée et forme ainsi un récipient dans la pièce amovible. L'ensemble est conçu en outre de façon à ce que l'on puisse faire varier la position de la pièce amovible entre une première position, où l'extrémité fermée de la pièce amovible se trouve à une première distance du fond du puits, et une seconde position, où l'extrémité fermée de la pièce amovible se situe à une seconde distance par rapport au fond du puits.

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