In vitro generation of gabaergic neurons from embryonic stem...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/0793 (2010.01) C12N 5/0735 (2010.01) C12N 5/079 (2010.01) A61K 35/30 (2006.01) C12Q 1/02 (2006.01)

Patent

CA 2537403

The present disclosure is directed to improved methods for efficiently producing neuroprogenitor cells and differentiated neural cells such as GABAergic neurons from pluripotent stem cells, for example embryonic stem cells. Using the disclosed methods, cell populations containing a high proportion of GABAergic neurons have been isolated. The neuroprogenitor cells and terminally differentiated cells of the present disclosure can be generated in large quantities, and therefore may serve as an excellent source for cell replacement therapy in neurodegenerative disorders and neuronal diseases such as stroke, ischemia, epilepsy, and Huntington's disease.

La présente invention se rapporte à des procédés perfectionnés permettant de produire efficacement des cellules neuro-progénitrices et des cellules nerveuses différenciées telles que des neurones GABAergiques à partir de cellules souches pluripotentes, par exemple des cellules souches embryonnaires. Les procédés décrits ont permis d'isoler des populations cellulaires contenant une proportion élevée de neurones GABAergiques. Les cellules neuro-progénitrices et les cellules à terminaison différenciée selon la présente invention peuvent être produites en grandes quantités et constituent ainsi une excellente source dans la thérapie de remplacement cellulaire pour les troubles neurodégénératifs et les maladies neuronales telles que les attaques, l'ischémie, l'épilepsie et la maladie de Huntington.

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